The purpose of this study was to compare the fatty acid profile of intramuscular fat (IMF) in semimembranosus, longissimus dorsi, ventral serratus, rectus abdominis, costal part of the diaphragm, and trapezius muscles in Large White and Landrace pigs. Samples were taken from pair carcasses of pigs at the Taurian Bacon slaughterhouse of ZAO “Freedom Farm Bacon”, Kherson. The fatty acid composition of the muscles of the Large White and Landrace pig breeds is similar to each other, especially in m. longisimus dorsi. The greatest differences in

fatty acid composition at the interbreed level were noted only for m. semimembranosus (p > 0.05, difference is not statistically significant). The lack of statistically significant differences in the composition of fatty acids in different muscles may be due to the insufficient sample size and genetic relatedness of the selected breeds. The largest, statistically significant differences were found in the content of fatty acids in the muscles of different groups, which is associated with the peculiarities of their structure and metabolism. Meat of Large White and Landrace pigs, grown in ZAO “Freedom Farm Bacon” in terms of iodine value met the requirements of processing technology, and the ratio of PUFA/SFA in muscle tissues exceeded the recommended norms by 2 times with overestimated values of the atherogenic index in m. semimembranosus.