Abstract Various serotypes of Actinobacillus pleuropneumonia are caused factors of porcine pleuropneumonia and lead to significant economic loss in whole word. Effective antibiotic therapy requires the data on individual serotype resistance and its coinfection variants. The aim of present study was to disclose A. pleuropneumonia epidemiology of swine farming in Ukraine during 2022–2024. The isolates from 16 provinces of Ukraine were analyzed with RT-PCR and microbial culture techniques to identify serotype and its antibiotic resistance. Four and five serotypes colocation observed only in 2 provinces. Besides, the infections caused by one serotype were observed in 79% cases, while coinfection cases of two and three A. pleuropneumoniae serotypes detected in 21% of all studied regions. Totally, 12 different serotypes were identified in all locations. The serotypes 8 and 2 were most prevailing and together consist 51% of all cases. The results on antibiotic resistance demonstrated significant diversity with respect to various antibiotic classes. However, isolated A. pleuropneumoniae strains exhibited high sensitivity to both β-lactams and quinolones while highest resistance detected with respect to macrolides and lincosamides. In spite of large spread of both 8 and 2 serotype, serotype 2 resistance to tetracyclines was significantly low and consists 2.8%. In contrast, serotype 8 resistance to tetracyclines made up 68.5%. Besides, inverse data on serotype 8 and 2 strains sensitivity to tetracyclines was detected as 13.9% and 80.6% respectively. Obtained data have shown that most of isolated strains were sensitive to cephalosporines, fluoroquinolones, and β-lactams while these strains exhibited high resistance with respect to macrolides and lincosamides. In addition, intermediate resistance was detected with respect to tetracyclines. Together, observed results evidence that multi-resistant A. pleuropneumoniae strains to macrolides, lincosamides, and tetracyclines could be generated in Ukraine recent years.