The current report presents a clinical case of a true vaginal prolapse complicated with unicornual uterine intussusception and anuria caused by urethral obstruction due to compression in a postpartum bitch. Transabdominal ultrasonographic examination showed no presence of retained fetuses into the birth canal, but an extremely enlarged urinary bladder. After repositioning of the protruded vaginal wall, laparotomy was performed, invagination of the right uterine horn into the uterus was observed, placed back to normal position, and spontaneous urination occurred. Ovariohysterectomy and cervicopexy to the abdominal wall for prevention of recurrence were done. The bitch had full recovery at one month follow-up. True vaginal prolapse in a bitch could be complicated with urethral obstruction due to compression, which may secondarily cause life-threatening azotemia. Reposition of the prolapsed vaginal tissue in the bitch is recommended to be followed by laparotomy in order to correct intussusception of the uterine horns and the body if presented.