In order to determine the influence of temperature seasonal factors on the productivity of goats and effect of their breed on the content of fatty acids in milk, an experiment was conducted with 30 goats of different genotypes throughout the year. The first group included 10 goats of the Saanen breed, the second group included 10 goats of the Russian white breed, the third group included 10 Ukrainian local breed goatsgoats of Ukrainian local selection. Productivity of goats as well as environmental temperature fluctuations were assessed daily, and the content of fatty acids in milk was determined twice a month during the year. Goats of the Saanen breed have a higher milk yield during the lactation period than goats of the Russian White breed and local goats of the Ukrainian selection. Genotype and external temperature influenced the milk yield of goats of all groups by 81.33% and 13.00%, respectively. It should also be noted that goats of the Saanen breed have a higher content of linolenic, isocaprylic, heptadecenoic, stearic, and erucic acids compared to goats of Russian white and Ukrainian local breed goatslocal Ukrainian selection. In Ukrainian local breed goatsgoats of local Ukrainian selection, the content of capric, lauric, arachinic, myristoleic, pentadecanoic, pentadecenoic, isopalmitic, palmitoleic, hexadecadienoic, linoleic, geneicosanoic acids was higher compared to goats of the Russian white breed and Saanen breeds. Russian white goats had a higher content of undecyl, behenic and docosahexaenoic acids compared to goats of Saanen breed Ukrainian local breed goatsand goats of local Ukrainian selection.